Glossary

pooled sample

A sample using cell hashing, where oligo-tagged antibodies against ubiquitously expressed surface proteins uniquely label cells from distinct samples, which can be subsequently pooled[SZHL+18] and be run as a single experiment.

preprocessing

To produce gene count matrix file, which can be directly used for an analysis pipeline. For example, in the case of pooled samples this means to retain either one gene count matrix file with each cell attributed to the donor of origin or to produce individual gene count matrix file for each donor

DAG

Directed Acyclic Graph. Click here for an example

SZHL+18

Marlon Stoeckius, Shiwei Zheng, Brian Houck-Loomis, Stephanie Hao, Bertrand Z Yeung, William M Mauck, 3rd, Peter Smibert, and Rahul Satija. Cell hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics. Genome Biol., 19(1):224, December 2018.